- Goals
- Clean and identify the muscles, nerves, and vessels of the gluteal region.
- Identify the greater sciatic foramen and the structures that traverse it.
- Clean and identify the muscles, nerve, and vessels of the posterior compartment of the thigh.
- Identify the boundaries and contents of the popliteal fossa.
Osteology Relevant to the Regions
Hip bones
Complete anatomy
Osteology of hip bones
Femur
Complete anatomy
Osteology of femur
■Head and Fovea capitis (for attachment of ligament of head)
■Neck
■Shaft
■Greater and lesser trochanters
■Intertrochanteric line & crest
■Trochanteric fossa
■Gluteal tuberosity—rough surface at the proximal end of the linea aspera, just below the lesser trochanter
■Linea aspera
■Popliteal surface
■Medial and lateral femoral condyles
Tibia
Complete anatomy
Osteology of tibia
Inspect a bony pelvis or the pelvic ligament model and review the attachments and locations of the:
■Sacrospinous ligament
■Sacrotuberous ligament
Question
Where is the greater sciatic foramen? Where is the lesser sciatic foramen?
Review the course and attachments of the piriformis and obturator internus muscles (remember them from the pelvis?).
Question
What course do the superior gluteal nerve and vessels take in order to leave the pelvic cavity? How are they related to the piriformis?
Question
What course do the inferior gluteal nerve and vessels & the sciatic nerve take in order to leave the pelvic cavity? How are they related to the piriformis?
Gluteal Region: Surface Anatomy
You will need to turn the dettached leg into the prone position so we can access the gluteal region and posterior thigh.
Before cutting, see if you can locate, observe, and/or palpate the following:
■Natal cleft (Intergluteal cleft)
■Gluteal fold
■Iliac crest
■Anterior superior and posterior superior iliac spines
■Greater trochanter
■Sacrum and coccyx
■Ischial tuberosity
■Popliteal fossa
■Head of fibula
Make skin incisions.
1Start at the posterior superior iliac spine (A) and make a curved incision forward along the iliac crest to the ASIS (B).
2Now make a curved incision from medial to lateral just below the gluteal fold (C to D).
3Reflect the skin flap over the gluteus maximus laterally.
1Now make a vertical incision from superior to inferior in the posterior thigh, starting at the gluteal fold above and ending below in the proximal calf
2Now make a horizontal incision just below the knee – stay SUPERFICIAL so you don’t cut small saphenous vein entering the popliteal fossa!
3Reflect the two flaps of skin in the posterior thigh medially and laterally.
Clean off gluteal muscles.
Complete anatomy
Superficial muscles of the gluteal region
1Remove the superficial fascia from the buttock in chunks.
There will be lots to remove! Clean all the superficial fascia away to reveal the deep fascia over the gluteus maximus muscle. It’s important that you clean enough away so that you can clearly visualize the superior and inferior borders of the gluteus maximus.
Note
This will be difficult along the inferior border of the gluteus maximus, where the gluteal region meets the posterior thigh.
Perseverance is the key!
2Above the superior border of the gluteus maximus will be some robust deep fascia that covers the gluteus medius. Open this fascia with a scalpel and scissors to better reveal the superior border of the gluteus maximus.
Clean superficial structures of posterior thigh.
Clean the copious superficial fascia from the posterior thigh to reveal the super tough fascia lata (deep fascia of the thigh) in the posterior thigh. The hamstring muscles are deep to this fascia.
Now, with scissors carefully open up and reflect the fascia lata in the posterior thigh, looking for the posterior cutaneous nerve of the thigh running down the midline of the posterior thigh, beginning above at the midpoint of the inferior border of the gluteus maximus and ending below in the posterior calf.
The posterior cutaneous nerve of the thigh is an oddball—it courses DEEP to the fascia lata, and sends small branches through the fascia lata into the overlying superficial fascia and skin.
Gluteal Region
Free up and then transect the gluteus maximus muscle.
1With blunt dissection, loosen up the superior border of the gluteus maximus muscle just below the iliac crest.
Question
What are the distal attachments (insertions) of the gluteus maximus? What is the main function of the gluteus maximus?
2With blunt dissection, create a plane between the gluteus maximus muscle and the underlying muscles beneath it.
Create this plane by sliding your hand beneath the gluteus maximus above its superior border, between the gluteus maximus and medius muscles.
3Transect the entire gluteus maximus from superior to inferior—from the midpoint of the superior border of the muscle to its inferior border where it crosses the ischial tuberosity.
Reflect the halves of the gluteus maximus and locate the inferior gluteal nerve and vessels entering the deep side of the muscle.
The key to the gluteal region is the piriformis muscle. Use blunt dissection to define the borders of the piriformis muscle as it emerges from the greater sciatic foramen to attach to the greater trochanter.
Below the piriformis, identify the inferior gluteal neurovascular bundle, the sciatic nerve, the posterior cutaneous nerve of the thigh, and most medially, the internal pudendal vessels and pudendal nerve.
Clinical puzzler
Divide the gluteal region into four quadrants: upper medial, upper lateral, lower medial and lower lateral. If you wanted to give a patient an intramuscular injection—which quadrant would be the best choice? (Injections should be done in safe places where there are no major nerves or vessels to injure.)
Review the course of the internal pudendal vessels and pudendal nerve as they pass from the pelvic cavity to the perineum
Clean and transect the gluteus medius.
3Then cut across the gluteus medius transversely several inches above the greater trochanter. Reflect the cut edges of the muscle to expose the gluteus minimus muscle.
Question
What are the attachments of the gluteus medius and minimus? Their actions?
What are the attachments of the gluteus medius and minimus? Their actions?
Clinical Pearl
The gluteus medius and gluteus minimus abduct the thigh at the hip. Additionally, these muscles are crucial to stabilizing the pelvis when you walk or raise one limb off the ground. When one limb is raised from the ground and the other limb bears the weight of the entire body above it, the gluteus medius and minimus contract to keep the contralateral pelvis from sagging (since the lower limb on the contralateral side is not in contact with the ground, and the pelvis would therefore have the tendency to sag).

Weakness or paralysis of the ipsilateral gluteus medius and minimus—the side with the limb planted on the ground—would present as sagging/drooping of the contralateral pelvis—the side with the limb raised off the ground. When tested, this sagging/drooping of the contralateral pelvis is called a Trendelenburg sign.
Clean and identify the external hip rotator muscles.
Complete anatomy
Deep muscles of the gluteal region
The tendon of the obturator internus muscle passes through the lesser sciatic foramen to attach to the greater trochanter. Where is the origin of the obturator internus?
The shiny, white tendon of the obturator internus muscle is sandwiched between two small, elongated muscles that lie in the horizontal plane: The gemelli muscles (the “twins”).
Locate the gemellus superior muscle and gemellus inferior muscle above and below the tendon of the obturator internus. The gemelli are small external rotators of the hip.
Identify the quadratus femoris muscle attaching to the ischial tuberosity and the greater trochanter. Look at the relationship between the quadratus femoris and the sciatic nerve.
With blunt dissection, separate the quadratus femoris from the inferior gemellus.
Summary—From superior to inferior, identify these external hip rotator muscles:
■Piriformis
■Superior gemellus
■Tendon of obturator internus
■Inferior gemellus
■Quadratus femoris
Posterior Thigh
Remove the remaining fascia lata from the thigh to reveal the posterior compartment.
Complete anatomy
Posterior thigh
With blunt dissection, separate the three muscles that make up the hamstrings = from lateral to medial these are the:
■Biceps femoris
■Semitendinosus
■Semimembranosus
Question
Why are they named this way?
Trace them to their insertions.
Question
What are the attachments of the hamstrings?
Note that they have a common proximal attachment, but their tendons diverge distally.
Question
Why is the lateral member of the hamstrings called the “biceps” femoris?
Review the anatomy of the pes anserinus. (See figures.) Although we haven’t dissected the anterior leg yet, you have heard of the pes, since it is the common distal attachment site for three muscles—two of which we examined in our last lab (sartorius and gracilis).
Question
Which of the hamstrings attaches to the pes?
Trace the sciatic nerve from the gluteal region distally as it passes through the posterior thigh into the popliteal fossa. Note that it passes deep to the long head of the biceps femoris.
The tibial part of the sciatic nerve innervates these muscles:
■Semitendinosus
■Semimembranosus
■Long head of the biceps femoris
■Hamstrings portion of the adductor magnus muscle
The common fibular part of the sciatic nerve supplies the short head of the biceps femoris.
Both nerves supply all the muscles in the lower extremity below the knee.
Usually in the mid-thigh is where the sciatic nerve splits into the tibial nerve and common fibular nerve.
This is variable; it may occur as high as the gluteal region or as low as the popliteal fossa.
Chalk Talk
Draw the sacral plexus and its branches - discuss the spinal levels that form the nerves seen in lab.
Complete anatomy
Lumbosacral plexus
Revisit the medial compartment to clean and identify structures related to the posterior thigh.
1Rotate the detached limb so that the medial compartment (work you did last lab) is visible. Clean the adductor magnus muscle and locate its two portions: adductor part and hamstrings part.
■Review their innervations.
2Clean and identify the adductor hiatus, the distal opening of the adductor canal.
■The femoral vessels change names as they exit the hiatus, becoming the popliteal artery and vein.
Popliteal Fossa
Carefully clean the superficial fascia from the popliteal fossa.
Complete anatomy
Structures in popliteal fossa
1Locate the small saphenous vein (ascending from the calf region) as it enters the popliteal fossa to terminate in the popliteal vein.
2Identify the borders of the diamond-shaped popliteal fossa:
■roof, the crural fascia (deep fascia of the leg)
■medial inferior border, the medial head of gastrocnemius
■lateral inferior border, the lateral head of gastrocnemius
■lateral superior border, the long head of the biceps femoris
■medial superior border, the semimembranosus
■floor, the popliteus muscle, popliteal surface of the femur, and the capsule of the knee joint
You won’t see the popliteus muscle today—but know that it is located in the floor of the popliteal fossa, hidden by the two heads of the gastrocnemius: It functions to “unlock” the fully extended knee joint, so that it can be flexed.
Question
Where are the tibial nerve and common fibular nerve positioned within the popliteal fossa?
3Using blunt dissection, identify from superficial to deep:
■Tibial nerve
■Popliteal vein
■Popliteal artery (the inferior continuation of the femoral artery after it passes through the adductor hiatus)
Checklist, Lab #17
Review and make sure you have identified each of the structures below.
Bones
See the list of bony landmarks in the osteology section at the top of this page.
Muscles—know where they attach and their innervations
Gluteus maximus, medius, and minimus
Tensor fasciae latae w/ Iliotibial tract
Piriformis
Obturator internus (tendon)
Superior and inferior gemellus muscles
Quadratus femoris
Biceps femoris: short and long heads
Semimembranosus
Semitendinosus
Adductor magnus (adductor and hamstring parts)
Other
Adductor hiatus—in adductor magnus muscle
Sacrotuberous and sacrospinous ligaments
Review boundaries of popliteal fossa
Nerves and Vessels
Superior gluteal artery and nerve
Inferior gluteal artery and nerve
Posterior cutaneous nerve of thigh
Sciatic nerve: Tibial and Common fibular nerves
Popliteal artery and vein
Small saphenous vein (see it entering the popliteal vein)




