Micro-ID topics
Sexually transmitted infections (STIs): Urethritis, cervicitis, vaginitis, and PID
High-yield summary Urethritis/Cervicitis: Syndromic approach Many are asymptomatic, especially in females Men Dysuria Thin mucoid discharge Pelvic pain Women Postcoital bleeding Mucopurulent discharge PID: look for cervical motion tenderness Think Chlamydia trachomatis (D-K) → most common cause Neisseria gonorrhoeae Less commonly: Mycoplasma genitalium Trichomonas HSV Chlamydia trachomatis (D-K) Key features Elementary body ↔ reticulate body […]
Sexually transmitted Infections (STIs): Genital ulcers and warts
High-yield summary Genital ulcer disease Key Differential A patient with a genital ulcer can usually be differentiated by: Disease Pain Ulcer Appearance Lymph Nodes Organism HSV Painful Multiple vesicles → shallow ulcers Tender HSV-2 most common for genital ulcers Primary syphilis Painless Single indurated chancre Nontender, rubbery Treponema pallidum Chancroid Very painful Soft ragged ulcer […]
Microbiome and antimicrobial stewardship
High-yield summary Microbiome basics Microbiome = all microbes + their genomes + the environment they occupy. Humans have ~38 trillion microbial cells (more than human cells). Major phyla: Firmicutes (Gram+ anaerobes) Bacteroidetes (Gram– anaerobes) Proteobacteria (Gram– facultative rods) Actinobacteria (Gram+ bacilli) Normal flora by site Carrier state: colonization with potential pathogen (e.g., S. aureus). […]
Key zoonoses
Naturally transmitted from animals to humans. Arthropod-borne bacteria and parasites Fleas Endemic typhus/murine typhus/Rickettsia typhi, epidemic typhus/Rickettsia prowazekii, Tunga penetrans Flies Sand Fly/Phlebotomus-cutaneous and visceral leishmaniasis, Tsetse Fly-African Sleeping Sickness/ T. brucei), Black Fly/Simulium-Onchocerciasis, Mango Fly/Chrysops-Loa Loa, Bot flies-Myiasis Mites/Chiggers Scrub typhus/Orientia tsutsugamushi, Scabies, Hair follicle mite/Demodex Mosquitos Malaria/Plasmodium, lymphatic filariasis/e.g. Wuchereria (many ARBO viruses […]
Notable bacterial toxins and mechanisms
These are exemplars of the most notable toxins and their mechanisms. Organisms such as Clostridia perfringens, Staphyloccoccus aureus, and Pseudomonas have a variety of toxins, some of which use similar mechanisms. Anthrax A/B toxin with three-protein components. Cell binding component is called protective antigen. There are two enzyme components: Edema factor Acts as adenylate cyclase […]
Infections based on predominant disease location (tropism)
Healthy Organisms associated with a healthy microbiome Bacteroides (GI tract) Lactobacillus (Vagina) Staphylococcus epidermidis (Skin) Head Central Nervous System (CNS) Meningitis: Differentiate by age (newborn, adult, elderly) and by “septic” (culture +) vs “aseptic” (culture negative – usually virus, prior antibiotics, or drug-induced). Bacteria: Neonate: E. coli, Group B Strep, Listeria Infant: Neisseria meningitidis, Strep […]
Outline of parasitic diseases and organisms according to site/specimen
Blood Plasmodium, Babesia, Trypanosoma, Microfilaria (Leishmania and Toxoplasma can be blood-bourne, but are not usually seen on the peripheral blood smear.). CSF Trypanosoma brucei, Naegleria, Angiostrongylus (eosinophilic meningitis). Brain Neurocysticercosis (Tinea solium), Toxoplasma. Eye Acanthamoeba, Toxoplasmosis, Onchocerciasis, Loa loa, visceral larva migrans (Toxocariasis). Mouth/Throat Ascaris. Sputum/Lung Ascaris, Strongyloides, Pneumocystis, Paragonimus. Abdomen/Ascites Echinococcal cysts, Entamoeba histolytica […]
Pharmacology: Introduction to antimicrobial therapy
High-yield summary Learning goals Understand five mechanisms of antibiotic action Differentiate empiric vs. directed therapy Recognize bacterial resistance mechanisms Apply PK/PD principles: bacteriostatic vs. bactericidal, time vs. concentration dependence Mechanisms of action Cell Wall Synthesis Inhibition: Penicillins, Cephalosporins, Carbapenems, Vancomycin. Protein Synthesis Inhibition: Aminoglycosides (30S), Macrolides, Clindamycin, Tetracyclines (50S). Cell Membrane Disruption: Daptomycin, Polymyxins. […]
Principles of microbiology laboratory diagnosis
High-yield summary Learning goals Compare direct examination, culture, serologic, and molecular methods for identifying microorganisms. Differentiate diagnostic approaches for bacteria vs. viruses. Understand how to select appropriate microbiologic diagnostic tests. Direct examination Uses stains (Gram, Acid-fast, fluorescent) to visualize organisms directly from specimens. Low sensitivity/specificity; risk of false positives/negatives. Gram stain: differentiates Gram-positive (purple) […]