If you read chapter 6: Bioenergetics and Oxidative Phosphorylation, from Lippincott, you should have no issue with the objectives. The short descriptions listed below are some of the very big picture, high yield points. The reading and the class session will go into more depth and detail. The reading guide is also posted. As I said in other modules, this reading guide is for people who have a difficult time focusing on these types of chapters. Most students DO NOT need to use the guide.
Diagram and describe how the mitochondria, via the electron transport chain, converts reducing power to chemical energy in the form of adenosine triphosphate (ATP), and describe how cells utilize ATP as energy
- Mitochondrial structure and why it is important
- Mitochondrial proteins
- Electron transport chain
- Redox pairs
- Glycerol-3-phosphate shuttle
- Malate-aspartate shuttle
Describe the mechanisms by which uncoupling proteins/agents, electron transport inhibitors, and ATP synthase inhibitors function in the mitochondria
- Amytal
- Rotenone
- Antimycin A
- Cyanide
- CO
- Sodium azide
- Uncoupling compounds (i.e. 2,4-dinitrophenol)
- Uncoupling proteins
Diagram and describe how the mitochondria, via the electron transport chain, converts reducing power to chemical energy in the form of adenosine triphosphate (ATP), and describe how cells utilize ATP as energy
This is one of the longer Osmosis videos I’ve seen, but I think it’s very well done. It covers part of the next learning goal as well (pay attention to UCP and uncoupling agents).
Describe the mechanisms by which uncoupling proteins/agents, electron transport inhibitors, and ATP synthase inhibitors function in the mitochondria
There are many different agents/inhibitors that will poison oxidative phosphorylation. The table below names a few and their site of inhibition.
| Inhibitor | Site of inhibition |
|---|---|
|
Rotenone, Amytal |
Transfer of electrons from complex I to coenzyme Q
|
|
Antimycin C |
Transfer of electrons from complex III to cytochrome c
|
|
Carbon monoxide (CO) |
Transfer of electrons from complex IV to oxygen |
|
Cyanide (CN) |
Transfer of electrons through complex IV to oxygen
|
|
Oligomycin |
Inhibits proton flow through the F0 component of the adenosine triphosphate (ATP) synthase
|
|
Dinitrophenol |
An uncoupler; facilitates proton transfer across the inner mitochondrial membrane
|
|
Thermogenin (UCP1) |
Decrease the proton gradient, increasing the permeability of the inner mitochondrial membrane (good for thermogenesis) |