Lab 3: Dissection: Shoulder and Pectoral Region

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  1. Study the scapula, clavicle, and proximal humerus and identify their named parts.
  2. Dissect the breast and identify its features in female cadavers.
  3. Reflect the skin and superficial fascia from the anterior and lateral thoracic wall.
  4. Clean and reflect the pectoral muscles and clean the serratus anterior muscle.
  5. Identify the nerves and vessels supplying the muscles in Goal #4.
  6. Bluntly dissect the axilla (armpit region) to identify the axillary vessels.
  7. Review the muscles of the posterior shoulder that move the scapula and upper limb.
  8. Identify the boundaries and contents of the quadrangular and triangular spaces.
  9. Identify the muscles of the rotator cuff and describe their functions.
  10. Identify the anatomic features of the glenohumeral (shoulder) joint.
  • As will be the case for all lab sessions, PLEASE COME TO LAB PREPARED!
  • You will have to work quickly and efficiently, as you need to identify the structures in the checklist during this session.
  • Please read this Dissector and look at Atlas figures BEFORE YOU COME TO LAB.
  • Work as a team in the peer teaching and learning mode during lab.
  • Unless otherwise instructed, follow the dissection instructions in this Dissector.

Osteology of the Shoulder Region

Scapula

On an isolated scapula, locate the following:

Borders of Scapula: Superior, Lateral, and Medial

Along the superior border, locate the scapular notch (contains the suprascapular nerve and vessels)

Inferior Angle

Supraspinous and Infraspinous Fossae

Subscapular fossa

Acromion

Coracoid process

Glenoid cavity

Supraglenoid and infraglenoid tubercles: for the attachment of the long head of the biceps brachii and the long head of the triceps brachii muscles, respectively.

Figure 3.1. Anterior view (left) and posterior view (right).

Clavicle

Figure 3.2. Right clavicle.

On an isolated clavicle, locate the following:

Sternal end

Acromial end

Shaft of clavicle. Is it straight? No! It has adouble curve”—do you see this?

Question

Divide the clavicle into thirds. In which portion are clavicle fractures most likely to occur? Why?

Humerus

On an isolated humerus, locate the following at the proximal end:

Head of humerus

Anatomical neck

Greater and lesser tubercles

Intertubercular (bicipital) groove

Question

Why is it called “bicipital”? [Hint: it contains a muscle tendon—which one?]

Surgical neck

Deltoid tuberosity

Groove for radial nerve

Figure 3.3. Proximal humerus.

Joints of the Shoulder Region

Identify these on the skeleton:

Sternoclavicular joint

Acromioclavicular joint

Glenohumeral joint

Scapulothoracic joint (is this a “true” joint?)

In your group, demonstrate and discuss the movements of the scapula:

Elevation

Depression

Protraction

Retraction

Upward rotation

Downward rotation

Figure 3.4. Scapulohumeral rhythm for full abduction.

Question

To achieve full 180-degree upper limb abduction at the glenohumeral joint, the scapula must rotate upward. Why?

Demonstrate and discuss the movements of the humerus at the glenohumeral (shoulder) joint:

Abduction

Adduction

Flexion

Extension

Circumduction

Medial (Internal) rotation

Lateral (External) rotation

Figure 3.5. Movements at glenohumeral joint.

Surface Anatomy: Inspection and Palpation

Your donor should be supine. Palpate and attempt to locate the following on your donor. Consult a skeleton for reference.

Figure 3.6.

Clavicle

Costal cartilages and costal margin

Sternum:

Suprasternal (jugular) notch

Manubrium

Sternal angle

Body

Xiphoid process

Acromion & coracoid processes of scapula

Figure 3.7.

Female donors: Inspect the breasts and note the nipple and areola.

Bumps on the areola in the living body are due to underlying sebaceous glands called areolar glands (Montgomery’s glands). They will be difficult to see in the donor.

Determine the extent of the base of the breast = Which ribs (numbers) and muscles does it overlie?

The breast has an axillary tail that extends into the armpit.

Dissect the breast in female donors

If you have a male, observe this dissection at another table. Do the breast dissection before removing the skin of the thorax.

Figure 3.8.

1Make a shallow incision in the skin around the areola, then make shallow sagittal and transverse incisions, dividing the skin over the breast into four quadrants.

2Reflect only the skin away from the areola, producing four skin flaps. Tug on the upper flaps in order to locate the fibrous suspensory ligaments (Coopers ligaments). These course between the fibroglandular tissue in the core of the breast and the overlying dermis. What is their function?

3Divide the breast into two equal halves with a deep sagittal incision through the center of the breast, down to the muscle.

4Look at the cut surfaces and identify the fibroglandular tissue in the center of the breast.

5Probe below the nipple to find lactiferous ducts.

6Place your finger under one of the hemi-breasts and create a plane between the pectoralis major muscle and breast itself = your finger is in the retromammary space. What is its functional and clinical significance?

7The dense fascia at the base of the breast superficial to your finger is retromammary fascia = this is part of the body wall’s superficial fascia.

8The fascia deep to your finger on the pectoralis major muscle is pectoral fascia. This is deep fascia—also called “investing” fascia.

9Clinicians divide the breast into quadrants (using vertical and horizontal lines through the nipple). These are called Upper Medial, Upper Lateral, Lower Medial, and Lower Lateral.

Question

Recalling that the breast has an axillary tail: which of the breast quadrants is largest and therefore has the most fibroglandular tissue? What bearing might this have on breast tumors?

Chalk Talk

The breast dissection may not be particularly satisfying. Be sure to do a chalk talk with your team concerning the female breast anatomy. The clinical significance of the breast warrants this.

Question

When discussing breast anatomy, consider its lymphatic drainage. Which lymph nodes receive most of the breast’s lymphatic drainage? What are sentinel nodes?

Reflect the skin (and breast) from the anterior and lateral thoracic wall
Figure 3.9.

1Make skin incisions as shown in the diagram, on both sides of the body. Cut into the superficial fascia, but not into the muscles.

2Carry incision 2 along the clavicle and over the shoulder into the upper arm.

3Carry incisions 3 and 4 to the midaxillary line (as close to the table top as possible). Incision 3 runs along the costal margin.

4Reflect the skin, superficial fascia, and breast together as flaps laterally. Leave the flaps attached to the body so they can be closed at the end of lab to keep the body moist.

5Clean fat and fascia from the pectoralis major muscles. Don’t worry if they aren’t squeaky clean.

Note

If your donor has a pacemaker implanted in the chest wall, notify an instructor so it can be removed properly. DO NOT cut the leads (wires) of the pacemaker.

You may see these while dissecting:

Anterior cutaneous branches of intercostal nerves and perforating branches of internal thoracic arteries poking out through the intercostal spaces just lateral to the sternum.

Lateral cutaneous branches of intercostal nerves and lateral cutaneous branches of posterior intercostal arteries poking out between the “serrations” of the serratus anterior muscle in the lateral chest wall.

These provide the segmental sensory nerve supply and segmental blood supply to the superficial tissues of the body wall.

Figure 3.10. Spinal nerve branches, superior view. NOTE THE SEGMENTATION.
Cut and reflect the pectoralis major and minor muscles

Complete anatomy

Pectoral region

Figure 3.11. Reflecting pectoralis major and pectoralis minor.

1Loosen the pectoralis major from the chest wall with blunt dissection (create an opening under the muscle with scissors along the inferior edge—then slide your fingers under the muscle through this opening to create a plane). With a scalpel or scissors cut the pectoralis major’s proximal attachments to the clavicle, sternum, and ribs. Reflect the muscle laterally toward the upper limb (the distal attachment of the muscle).

2Cut the pectoralis minor’s proximal attachment to the ribs and reflect the muscle superiorly toward its distal attachment to the coracoid process of the scapula.

3Carefully clean the neurovascular bundles (nerve, artery, and vein) that enter the deep surfaces of the muscles.

Note

Nerves and vessels usually enter the deep surfaces of muscles where they are protected.

Identify:

Lateral pectoral nerve (with pectoral artery and vein): courses above the superior border of pectoralis minor, then into the deep surface of pectoralis major.

Medial pectoral nerve: penetrates (and innervates) the pectoralis minor first, then passes into pectoralis major. Therefore, the pec major is innervated by BOTH pectoral nerves.

Question

What the heck? The medial and lateral pectoral nerves don’t seem aptly named. Why are they named this way???

These nerves originate from the brachial plexus (the nerve plexus supplying the upper limb). The medial pectoral nerve arises from the medial cord of the brachial plexus; the lateral pectoral nerve from the lateral cord. Makes perfect sense, eh?
Clean the axilla with blunt dissection and scissors

In the next lab session we will be studying the axillary region in detail. However, if you have time today you should start working in this area to find some of the structures related to the shoulder.

1The axilla (armpit) is the pyramid-shaped space under the shoulder joint, where the upper limb meets the trunk. The nerves and vessels to the upper limb pass through the axilla. Abduct the donor’s upper limb as far laterally as possible. Use your fingers to loosen up the axilla. The fascia and fatty tissue here are dense and thick and must be separated with scissors (DON’T CUT—instead use the open-and-close method)!

2Clean the lateral wall of the chest (this is the medial wall of the axilla) and identify the serratus anterior muscle. Note its “serrated” attachments to the ribs. This muscle depresses and protracts the scapula, keeping it flush against the body wall.

3Clean and identify the long thoracic nerve coursing along the surface of the serratus anterior. It passes from superior to inferior. Its origin is in the root of the neck, above the axilla, from the brachial plexus (spinal nerves C-5, C-6, and C-7).

Clinical correlation

The course of the long thoracic nerve on the external surface of the muscle is an exception. This makes the nerve vulnerable to injury. Paralysis of the muscle (damage to the long thoracic nerve) could cause the medial border of the scapula to protrude posteriorly—a condition called “winged scapula.”

Figure 3.12.

4Clean the axillary artery and vein. What is their relationship to the pectoralis minor muscle? Carefully clean the artery in order to identify two of its branches that supply muscles in the pectoral region:

    • the thoraco-acromial trunk = this short vessel gives off pectoral arteries that supply the pec major and minor muscles
    • the lateral thoracic artery = it travels in tandem with the long thoracic nerve to supply the serratus anterior muscle. Don’t get the lateral thoracic artery and long thoracic nerve confused!

5Look for axillary lymph nodes in the fascia surrounding the axillary vein. If your donor has prominent or enlarged nodes, notify other groups in the lab to come and have a look. Axillary nodes are important, as they receive lymph from the entire upper limb, the breast, and all the superficial tissues of the trunk wall above the umbilicus (“belly button”). That’s a lot of territory!

Prosections: Rotator Cuff and Glenohumeral Joint

There are three prosections you should review to study the anatomy of the posterior shoulder, rotator cuff, and glenohumeral joint. An instructor will demonstrate these to groups of students.

Muscles of the Posterior Shoulder

Complete anatomy

Muscles of the shoulder

These muscles move the scapula and upper limb. You saw many of these muscles in the previous lab session, but now is a good time to review these in detail, particularly their role in movement of the shoulder.

On the superficial side of the dissection, identify the following muscles:

Trapezius

Trace out its upper portion (descending fibers), middle portion (transverse fibers), and inferior portion (ascending fibers).

Question

What are the functions of these parts of the muscle?

Figure 3.13.

Latissimus dorsi

Posterior part of the deltoid muscle

Triceps brachii—this muscle is in the posterior arm.

The lateral head and long head of the triceps should be visible.

Know the attachments, actions, and innervations of the muscles listed above.

On the deep side of the dissection, identify the following muscles:

Rhomboid muscles (major and minor)

Levator scapulae

Supraspinatus

Infraspinatus

Teres minor

Teres major

Know the attachments, actions, and innervations of the muscles listed above.

Figure 3.14.

Complete anatomy

Neurovasculature tracts of the scapula

Geometry fans! Identify these spaces in the posterior shoulder—they transmit nerves and vessels from the axilla to the posterior shoulder. These spaces can only be identified when the posterior part of the deltoid is detached and elevated.

Figure 3.15.

Quadrangular space:

Superior border = teres minor muscle

Lateral border =humerus

Medial border = long head of triceps brachii

Inferior border = teres major muscle

The axillary nerve and posterior circumflex humeral artery traverse the quadrangular space.

 

Triangular space:

Superior border = teres minor muscle

Lateral border = long head of triceps brachii

Inferior border = teres major muscle

The circumflex scapular artery traverses the triangular space.

Figure 3.16.

Rotator Cuff

On the disarticulated upper limb specimen, identify the four muscles that make up the rotator cuff:

Supraspinatus

Infraspinatus

Teres minor

Subscapularis

Know the attachments, actions, and innervations of these muscles listed.

Question

Why is this group of muscles referred to as the “rotator cuff”?

question

Besides external and internal rotation, what is the important function of this muscle group with regards to the glenohumeral joint?

Figure 3.17.
Figure 3.18.

The posterior compartment of the arm (containing the triceps brachii) will be visible on this specimen as well. See if you can identify the three heads of the triceps: long, lateral, and medial (some anatomists argue that the medial head should be called the “deep” head).

Figure 3.19.
Figure 3.20.

Glenohumeral (Shoulder) Joint

Complete anatomy

Glenohumeral joint

Review the bony anatomy of the glenohumeral joint:

Head of humerus

Glenoid cavity of scapula

Figure 3.21.
Figure 3.22.

question

The bony surfaces of the glenohumeral joint have poor congruence. Is the humeral head (ball) well-covered by the glenoid cavity (socket)? How does this affect joint stability?

If a prosected specimen is available with the glenohumeral joint opened (the joint capsule has been opened), identify the following:

Articular cartilage on the humeral head and in the glenoid cavity —is it smooth?

Glenoid labrum—what is its function?

The long head of the biceps brachii muscle passes through the glenohumeral joint cavity. Odd! It inserts onto the supraglenoid tubercle.

Checklist, Lab #3

Review and make sure you have identified each of the structures below.

Scapula

Borders of the scapula: superior, medial, lateral

Inferior Angle

Supraspinous and Infraspinous Fossae

Subscapular fossa

Acromion

Coracoid process

Glenoid cavity

Supraglenoid and infraglenoid tubercles

Proximal humerus

Head of humerus

Anatomical neck

Greater and lesser tubercles

Intertubercular (bicipital) groove

Surgical neck

Deltoid tuberosity

Groove for radial nerve

Surface landmarks

Clavicle

Suprasternal notch

Sternal angle

Costal cartilages

Costal margin

Breast

Nipple and areola

Fibroglandular tissue

Lactiferous ducts

Suspensory (Cooper’s) ligaments

Retromammary space

Pectoral Region and Axilla

Pectoralis major and minor muscles

Serratus anterior muscle

Medial and lateral pectoral nerves

Long thoracic nerve

Axillary artery and vein

Thoraco-acromial trunk (artery) with pectoral arteries

Lateral thoracic artery and vein

Axillary lymph nodes

Posterior shoulder (Prone prosection)

Trapezius

Latissimus dorsi

Rhomboid muscles (major/minor)

Levator scapulae

Deltoid (posterior portion)

Teres major

Triceps brachii (long, lateral, and medial head)

Quadrangular space (w/ axillary n. & posterior circumflex humeral a.)

Triangular space (w/ circumflex scapular a.)

Rotator cuff (Isolated limb)

Supraspinatus

Infraspinatus

Teres minor

Subscapularis

Glenohumeral joint prosection (if available)

Glenoid labrum

Long head of biceps brachii

Table of Contents
Headshot of David Conley, PhD · Clinical Associate Professor, Department of Translational Medicine & Physiology
David Conley
PhD · Clinical Associate Professor, Department of Translational Medicine & Physiology
Office: PBS 41A
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Headshot of Shannon Helbling, PhD · Clinical Assistant Professor, Department of Translational Medicine & Physiology
Shannon Helbling
PhD · Clinical Assistant Professor, Department of Translational Medicine & Physiology
Office: PBS 41C
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