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- Goals
- Pre-lab preparation
- Study the scapula, clavicle, and proximal humerus and identify their named parts.
- Dissect the breast and identify its features in female cadavers.
- Reflect the skin and superficial fascia from the anterior and lateral thoracic wall.
- Clean and reflect the pectoral muscles and clean the serratus anterior muscle.
- Identify the nerves and vessels supplying the muscles in Goal #4.
- Bluntly dissect the axilla (armpit region) to identify the axillary vessels.
- Review the muscles of the posterior shoulder that move the scapula and upper limb.
- Identify the boundaries and contents of the quadrangular and triangular spaces.
- Identify the muscles of the rotator cuff and describe their functions.
- Identify the anatomic features of the glenohumeral (shoulder) joint.
- As will be the case for all lab sessions, PLEASE COME TO LAB PREPARED!
- You will have to work quickly and efficiently, as you need to identify the structures in the checklist during this session.
- Please read this Dissector and look at Atlas figures BEFORE YOU COME TO LAB.
- Work as a team in the peer teaching and learning mode during lab.
- Unless otherwise instructed, follow the dissection instructions in this Dissector.
Osteology of the Shoulder Region
Scapula
Scapula
On an isolated scapula, locate the following:
■Borders of Scapula: Superior, Lateral, and Medial
■Along the superior border, locate the scapular notch (contains the suprascapular nerve and vessels)
■Inferior Angle
■Supraspinous and Infraspinous Fossae
■Subscapular fossa
■Acromion
■Coracoid process
■Glenoid cavity
■Supraglenoid and infraglenoid tubercles: for the attachment of the long head of the biceps brachii and the long head of the triceps brachii muscles, respectively.
Clavicle
Clavicle
Question
Divide the clavicle into thirds. In which portion are clavicle fractures most likely to occur? Why?
Humerus
Humerus
On an isolated humerus, locate the following at the proximal end:
■Head of humerus
■Anatomical neck
■Greater and lesser tubercles
■Intertubercular (bicipital) groove
Question
Why is it called “bicipital”? [Hint: it contains a muscle tendon—which one?]
■Surgical neck
■Deltoid tuberosity
■Groove for radial nerve
Joints of the Shoulder Region
Identify these on the skeleton:
■Sternoclavicular joint
■Acromioclavicular joint
■Glenohumeral joint
■Scapulothoracic joint (is this a “true” joint?)
Question
To achieve full 180-degree upper limb abduction at the glenohumeral joint, the scapula must rotate upward. Why?
Surface Anatomy: Inspection and Palpation
Your donor should be supine. Palpate and attempt to locate the following on your donor. Consult a skeleton for reference.

Female donors: Inspect the breasts and note the nipple and areola.
■Bumps on the areola in the living body are due to underlying sebaceous glands called areolar glands (Montgomery’s glands). They will be difficult to see in the donor.
■Determine the extent of the base of the breast = Which ribs (numbers) and muscles does it overlie?
■The breast has an axillary tail that extends into the armpit.
Breast
Dissect the breast in female donors
If you have a male, observe this dissection at another table. Do the breast dissection before removing the skin of the thorax.

1Make a shallow incision in the skin around the areola, then make shallow sagittal and transverse incisions, dividing the skin over the breast into four quadrants.
2Reflect only the skin away from the areola, producing four skin flaps. Tug on the upper flaps in order to locate the fibrous suspensory ligaments (Cooper’s ligaments). These course between the fibroglandular tissue in the core of the breast and the overlying dermis. What is their function?
3Divide the breast into two equal halves with a deep sagittal incision through the center of the breast, down to the muscle.
4Look at the cut surfaces and identify the fibroglandular tissue in the center of the breast.
5Probe below the nipple to find lactiferous ducts.
6Place your finger under one of the hemi-breasts and create a plane between the pectoralis major muscle and breast itself = your finger is in the retromammary space. What is its functional and clinical significance?
7The dense fascia at the base of the breast superficial to your finger is retromammary fascia = this is part of the body wall’s superficial fascia.
8The fascia deep to your finger on the pectoralis major muscle is pectoral fascia. This is deep fascia—also called “investing” fascia.
9Clinicians divide the breast into quadrants (using vertical and horizontal lines through the nipple). These are called Upper Medial, Upper Lateral, Lower Medial, and Lower Lateral.
Question
Recalling that the breast has an axillary tail: which of the breast quadrants is largest and therefore has the most fibroglandular tissue? What bearing might this have on breast tumors?
Chalk Talk
The breast dissection may not be particularly satisfying. Be sure to do a chalk talk with your team concerning the female breast anatomy. The clinical significance of the breast warrants this.
Question
When discussing breast anatomy, consider its lymphatic drainage. Which lymph nodes receive most of the breast’s lymphatic drainage? What are sentinel nodes?
Reflect the skin (and breast) from the anterior and lateral thoracic wall

1Make skin incisions as shown in the diagram, on both sides of the body. Cut into the superficial fascia, but not into the muscles.
2Carry incision 2 along the clavicle and over the shoulder into the upper arm.
3Carry incisions 3 and 4 to the mid–axillary line (as close to the table top as possible). Incision 3 runs along the costal margin.
4Reflect the skin, superficial fascia, and breast together as flaps laterally. Leave the flaps attached to the body so they can be closed at the end of lab to keep the body moist.
5Clean fat and fascia from the pectoralis major muscles. Don’t worry if they aren’t squeaky clean.
Note
If your donor has a pacemaker implanted in the chest wall, notify an instructor so it can be removed properly. DO NOT cut the leads (wires) of the pacemaker.
You may see these while dissecting:
■Anterior cutaneous branches of intercostal nerves and perforating branches of internal thoracic arteries poking out through the intercostal spaces just lateral to the sternum.
■Lateral cutaneous branches of intercostal nerves and lateral cutaneous branches of posterior intercostal arteries poking out between the “serrations” of the serratus anterior muscle in the lateral chest wall.
These provide the segmental sensory nerve supply and segmental blood supply to the superficial tissues of the body wall.
Cut and reflect the pectoralis major and minor muscles
Pectoral region

1Loosen the pectoralis major from the chest wall with blunt dissection (create an opening under the muscle with scissors along the inferior edge—then slide your fingers under the muscle through this opening to create a plane). With a scalpel or scissors cut the pectoralis major’s proximal attachments to the clavicle, sternum, and ribs. Reflect the muscle laterally toward the upper limb (the distal attachment of the muscle).
2Cut the pectoralis minor’s proximal attachment to the ribs and reflect the muscle superiorly toward its distal attachment to the coracoid process of the scapula.
3Carefully clean the neurovascular bundles (nerve, artery, and vein) that enter the deep surfaces of the muscles.
Note
Nerves and vessels usually enter the deep surfaces of muscles where they are protected.
Identify:
■Lateral pectoral nerve (with pectoral artery and vein): courses above the superior border of pectoralis minor, then into the deep surface of pectoralis major.
■Medial pectoral nerve: penetrates (and innervates) the pectoralis minor first, then passes into pectoralis major. Therefore, the pec major is innervated by BOTH pectoral nerves.
Question
What the heck? The medial and lateral pectoral nerves don’t seem aptly named. Why are they named this way???
Clean the axilla with blunt dissection and scissors
In the next lab session we will be studying the axillary region in detail. However, if you have time today you should start working in this area to find some of the structures related to the shoulder.
1The axilla (armpit) is the pyramid-shaped space under the shoulder joint, where the upper limb meets the trunk. The nerves and vessels to the upper limb pass through the axilla. Abduct the donor’s upper limb as far laterally as possible. Use your fingers to loosen up the axilla. The fascia and fatty tissue here are dense and thick and must be separated with scissors (DON’T CUT—instead use the open-and-close method)!
2Clean the lateral wall of the chest (this is the medial wall of the axilla) and identify the serratus anterior muscle. Note its “serrated” attachments to the ribs. This muscle depresses and protracts the scapula, keeping it flush against the body wall.
3Clean and identify the long thoracic nerve coursing along the surface of the serratus anterior. It passes from superior to inferior. Its origin is in the root of the neck, above the axilla, from the brachial plexus (spinal nerves C-5, C-6, and C-7).
Clinical correlation
The course of the long thoracic nerve on the external surface of the muscle is an exception. This makes the nerve vulnerable to injury. Paralysis of the muscle (damage to the long thoracic nerve) could cause the medial border of the scapula to protrude posteriorly—a condition called “winged scapula.”
4Clean the axillary artery and vein. What is their relationship to the pectoralis minor muscle? Carefully clean the artery in order to identify two of its branches that supply muscles in the pectoral region:
-
- the thoraco-acromial trunk = this short vessel gives off pectoral arteries that supply the pec major and minor muscles
- the lateral thoracic artery = it travels in tandem with the long thoracic nerve to supply the serratus anterior muscle. Don’t get the lateral thoracic artery and long thoracic nerve confused!
5Look for axillary lymph nodes in the fascia surrounding the axillary vein. If your donor has prominent or enlarged nodes, notify other groups in the lab to come and have a look. Axillary nodes are important, as they receive lymph from the entire upper limb, the breast, and all the superficial tissues of the trunk wall above the umbilicus (“belly button”). That’s a lot of territory!
Prosections: Rotator Cuff and Glenohumeral Joint
There are three prosections you should review to study the anatomy of the posterior shoulder, rotator cuff, and glenohumeral joint. An instructor will demonstrate these to groups of students.
Muscles of the Posterior Shoulder
Muscles of the shoulder
These muscles move the scapula and upper limb. You saw many of these muscles in the previous lab session, but now is a good time to review these in detail, particularly their role in movement of the shoulder.
On the superficial side of the dissection, identify the following muscles:
■Latissimus dorsi
■Posterior part of the deltoid muscle
■Triceps brachii—this muscle is in the posterior arm.
■The lateral head and long head of the triceps should be visible.
Know the attachments, actions, and innervations of the muscles listed above.
On the deep side of the dissection, identify the following muscles:
Neurovasculature tracts of the scapula
Geometry fans! Identify these spaces in the posterior shoulder—they transmit nerves and vessels from the axilla to the posterior shoulder. These spaces can only be identified when the posterior part of the deltoid is detached and elevated.

Quadrangular space:
■Superior border = teres minor muscle
■Lateral border =humerus
■Medial border = long head of triceps brachii
■Inferior border = teres major muscle
■The axillary nerve and posterior circumflex humeral artery traverse the quadrangular space.
Triangular space:
■Superior border = teres minor muscle
■Lateral border = long head of triceps brachii
■Inferior border = teres major muscle
■The circumflex scapular artery traverses the triangular space.
Rotator Cuff
Rotator cuff
On the disarticulated upper limb specimen, identify the four muscles that make up the rotator cuff:
■Supraspinatus
■Infraspinatus
■Teres minor
■Subscapularis
Know the attachments, actions, and innervations of these muscles listed.
Question
Why is this group of muscles referred to as the “rotator cuff”?
question
Besides external and internal rotation, what is the important function of this muscle group with regards to the glenohumeral joint?
The posterior compartment of the arm (containing the triceps brachii) will be visible on this specimen as well. See if you can identify the three heads of the triceps: long, lateral, and medial (some anatomists argue that the medial head should be called the “deep” head).
Glenohumeral (Shoulder) Joint
Glenohumeral joint
Review the bony anatomy of the glenohumeral joint:
■Head of humerus
■Glenoid cavity of scapula
question
The bony surfaces of the glenohumeral joint have poor congruence. Is the humeral head (ball) well-covered by the glenoid cavity (socket)? How does this affect joint stability?
If a prosected specimen is available with the glenohumeral joint opened (the joint capsule has been opened), identify the following:
■Articular cartilage on the humeral head and in the glenoid cavity —is it smooth?
■Glenoid labrum—what is its function?
■The long head of the biceps brachii muscle passes through the glenohumeral joint cavity. Odd! It inserts onto the supraglenoid tubercle.
Checklist, Lab #3
Review and make sure you have identified each of the structures below.
Scapula
Borders of the scapula: superior, medial, lateral
Inferior Angle
Supraspinous and Infraspinous Fossae
Subscapular fossa
Acromion
Coracoid process
Glenoid cavity
Supraglenoid and infraglenoid tubercles
Proximal humerus
Head of humerus
Anatomical neck
Greater and lesser tubercles
Intertubercular (bicipital) groove
Surgical neck
Deltoid tuberosity
Groove for radial nerve
Surface landmarks
Clavicle
Suprasternal notch
Sternal angle
Costal cartilages
Costal margin
Breast
Nipple and areola
Fibroglandular tissue
Lactiferous ducts
Suspensory (Cooper’s) ligaments
Retromammary space
Pectoral Region and Axilla
Pectoralis major and minor muscles
Serratus anterior muscle
Medial and lateral pectoral nerves
Long thoracic nerve
Axillary artery and vein
Thoraco-acromial trunk (artery) with pectoral arteries
Lateral thoracic artery and vein
Axillary lymph nodes
Posterior shoulder (Prone prosection)
Trapezius
Latissimus dorsi
Rhomboid muscles (major/minor)
Levator scapulae
Deltoid (posterior portion)
Teres major
Triceps brachii (long, lateral, and medial head)
Quadrangular space (w/ axillary n. & posterior circumflex humeral a.)
Triangular space (w/ circumflex scapular a.)
Rotator cuff (Isolated limb)
Supraspinatus
Infraspinatus
Teres minor
Subscapularis
Glenohumeral joint prosection (if available)
Glenoid labrum
Long head of biceps brachii